Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments
Xu, MY; Chen, XJ; Qiu, MD; Zeng, XW; Xu, J; Deng, DY; Sun, GP; Li, X; Guo, J; xumy@gdim.cn; guojun@gzb.ac.cn
2012
发表期刊PLOS ONE
ISSN1932-6203
卷号7期号:1页码:e30439
摘要Polybrominated diphenyl ethers (PBDEs) can be reductively degraded by microorganisms under anaerobic conditions. However, little is known about the effect of electron donors on microbial communities involved in PBDEs degradation. Here we employed 454 Titanium pyrosequencing to examine the phylogenetic diversity, composition, structure and dynamics of microbial communities from microcosms under the conditions of different electron donor amendments. The community structures in each of the five alternate electron donor enrichments were significantly shifted in comparison with those of the control microcosm. Commonly existing OTUs between the treatment and control consortia increased from 5 to 17 and more than 50% of OTUs increased around 13.7 to 186 times at least in one of the microcosms after 90-days enrichment. Although the microbial communities at different taxonomic levels were significantly changed by different environmental variable groups in redundancy analysis, significant correlations were observed between the microbial communities and PBDE congener profiles. The lesser-brominated PBDE congeners, tri-BDE congener (BDE-32) and hexa-BDE, were identified as the key factors shaping the microbial community structures at OTU level. Some rare populations, including the known dechlorinating bacterium, Dehalobacter, showed significant positive-correlation with the amounts of PBDE congeners in the consortia. The same results were also observed on some unclassified bacteria. These results suggest that PBDEs-degrading microbial communities can be successfully enriched, and their structures and compositions can be manipulated through adjusting the environmental parameters.
部门归属[Xu, Meiying; Chen, Xingjuan; Qiu, Mengde; Deng, Daiyong; Sun, Guoping; Guo, Jun] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangzhou, Guangdong, Peoples R China; [Zeng, Xiaowei; Xu, Jian] CAS, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China; [Zeng, Xiaowei; Xu, Jian] CAS, Bioenergy Genome Ctr, Qingdao, Shandong, Peoples R China; [Xu, Meiying; Chen, Xingjuan; Deng, Daiyong; Sun, Guoping; Guo, Jun] Minist Guangdong Prov Jointly Breeding Base, State Key Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China; [Xu, Meiying; Chen, Xingjuan; Deng, Daiyong; Sun, Guoping; Guo, Jun] Guangdong Open Lab Appl Microbiol, Guangzhou, Guangdong, Peoples R China; [Li, Xiang] CAS, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China
学科领域Multidisciplinary Sciences
语种英语
资助项目LMB
WOS记录号WOS:000301640600031
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9988
专题中科院海洋生物资源可持续利用重点实验室
通讯作者xumy@gdim.cn; guojun@gzb.ac.cn
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GB/T 7714
Xu, MY,Chen, XJ,Qiu, MD,et al. Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments[J]. PLOS ONE,2012,7(1):e30439.
APA Xu, MY.,Chen, XJ.,Qiu, MD.,Zeng, XW.,Xu, J.,...&guojun@gzb.ac.cn.(2012).Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments.PLOS ONE,7(1),e30439.
MLA Xu, MY,et al."Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments".PLOS ONE 7.1(2012):e30439.
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